Induction of immune responses after intragastric administration of 1 mice with Lactobacillus casei producing porcine parvovirus 2 protective antigen VP
نویسندگان
چکیده
Lactobacillus casei ATCC 393 was selected as an antigen delivery vehicle for 6 mucosal immunization against porcine parvovirus (PPV) infection. A 64kDa fragment of PPV 7 major protective antigen VP2 protein was used as the parvovirus antigen model. A 8 recombinant lactobacilli expressing VP2 protein was constructed with plasmid pPG611.1, 9 where expression and localization of the VP2 protein from the recombinant Lc393-rPPV-VP2 10 was detected via SDS-PAGE, Western blot and immunofluorescence. BALB/c mice oral 11 immunized with the recombinant lactobacilli expressing VP2 protein were induced both local 12 mucosal and systemic immune responses against PPV. The induced antibodies demonstrated 13 neutralizing effects on PPV infection. These data indicated that the recombinant lactobacilli 14 could be a valuable strategy for future vaccine development of PPV. 15
منابع مشابه
Immunogenicity of recombinant classic swine fever virus CD8(+) T lymphocyte epitope and porcine parvovirus VP2 antigen coexpressed by Lactobacillus casei in swine via oral vaccination.
Classical swine fever virus (CSFV) and porcine parvovirus (PPV) are highly contagious pathogens, resulting in enormous economic losses in pig industries worldwide. Because vaccines play an important role in disease control, researchers are seeking improved vaccines that could induce antiviral immune responses against CSFV and PPV at the mucosal and systemic levels simultaneously. In this study,...
متن کاملEnhanced immunological memory responses to Listeria monocytogenes in rodents, as measured by delayed-type hypersensitivity (DTH), adoptive transfer of DTH, and protective immunity, following Lactobacillus casei Shirota ingestion.
We have investigated the effect of orally administered Lactobacillus casei Shirota (L. casei) on immunological memory, as measured by delayed-type hypersensitivity (DTH) and acquired cellular resistance (ACR). The studies were performed in animal models in which the animals were rendered immune by a primary Listeria monocytogenes infection. It was shown that orally administered viable L. casei,...
متن کاملProtective effect of Lactobacillus casei strain Shirota on Shiga toxin-producing Escherichia coli O157:H7 infection in infant rabbits.
We examined colonization patterns of Shiga toxin-producing Escherichia coli (STEC), concentrations of Shiga toxins (Stxs) and specific immunoglobulin A (lgA) against Stxs and STEC bacterial cell surface antigen in various portions of the gastrointestinal tract in an infant rabbit infection model. After inoculation of 3-day-old infant rabbits with STEC strain 89020087 at low doses (approximately...
متن کاملInduction of immune responses in mice after oral immunization with recombinant Lactobacillus casei strains expressing enterotoxigenic Escherichia coli F41 fimbrial protein.
In an effort to develop a safe and effective vaccine for the prevention of enterotoxigenic Escherichia coli (ETEC) F41 infections, we have developed a surface antigen display system using poly-gamma-glutamate synthetase A (PgsA) as an anchoring matrix. The recombinant fusion proteins comprised of PgsA and fimbrial protein of F41 were stably expressed in Lactobacillus casei 525. Surface localiza...
متن کاملOral Immunization against PEDV with Recombinant Lactobacillus casei Expressing Dendritic Cell-Targeting Peptide Fusing COE Protein of PEDV in Piglets
Porcine epidemic diarrhea (PED) is a highly contagious disease in newborn piglets. In our previous study, a genetically engineered Lactobacillus casei oral vaccine (pPG-COE-DCpep/L393) expressing a dendritic cell (DC)-targeting peptide fused with porcine epidemic diarrhea virus (PEDV) COE antigen was developed. This vaccine induced significant levels of anti-PEDV specific IgG and IgA antibody r...
متن کامل